P150 CAF1 Rabbit Monoclonal Antibody
Product Specifications
Background
Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
Product Name Alternative
CAF; CAF1; CAF1P150; CHAF1A; DCAF1; hp15; P150; ; CAF 1 subunit A
Gene Name
P150 CAF1
Swiss Prot
Q13111
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Conjugation
Unconjugated
Applications
WB, IHC, ICC/IF, ICC, FC, IP
Purification
Affinity Purification
Dilution
WB 1:1000-1:2000, IHC 1:100-1:200, ICC/IF 1:50-1:200, ICC 1:50-1:200, FC 1:20-1:100, IP 1:20-1:50
Form
Liquid
Buffer
Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.
Modification
Unmodified
Molecular Weight
Calculated MW: 107 kDa ; Observed MW: 150 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Isotype
IgG
Available Sizes
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