CDK8 (2S1) Rabbit Monoclonal Antibody
Product Specifications
Background
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C- terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
Product Name Alternative
CDK8 protein kinase; Cell division protein kinase 8; Cyclin Dependent kinase 8; K35; Mediator complex subunit cdk8; Mediator of RNA polymerase II transcription subunit cdk8; Protein kinase K35
Gene Name
CDK8
Gene ID
1024
Swiss Prot
P49336
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Conjugation
Unconjugated
Applications
WB, IP
Purification
Affinity purification
Dilution
WB 1:500-1:2000, IP 1:20-1:50
Form
Liquid
Buffer
Supplied in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Modification
Unmodified
Molecular Weight
53kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.
Isotype
IgG
Available Sizes
Curated Selection
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