Caspase-9 (13Z10) Rabbit Monoclonal Antibody

Product Specifications

Background

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly (ADP-ribose) polymerase (PARP) .

Product Name Alternative

APAF-3; Caspase9; ICE-LAP6; Apoptotic protease Mch-6; CASP-9; Caspase-9; Caspase-9 precursor; MCH6; RNCASP9

Gene Name

CASP9

Gene ID

842

Swiss Prot

P55211

Host

Rabbit

Reactivity

Human

Clonality

Monoclonal

Conjugation

Unconjugated

Applications

WB, IHC, ICC/IF, FC, IP

Purification

Affinity purification

Dilution

WB 1:1000-1:5000, IHC 1:50-1:200, ICC/IF 1:200-1:500, FC 1:100-1:200, IP 1:50-1:100

Form

Liquid

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.