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Firefly Luciferase (13Z19) Rabbit Monoclonal Antibody

Product Specifications

Background

Produces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. Produces green light with a wavelength of 562 nm.

Product Name Alternative

Luciferase; Firefly; Luciferin 4 monooxygenase

Gene Name

LUCI

Swiss Prot

P08659

Host

Rabbit

Reactivity

Other

Clonality

Monoclonal

Conjugation

Unconjugated

Applications

WB, ICC/IF, FC

Purification

Affinity purification

Dilution

WB 1:2000-1:10000, ICC/IF 1:200-1:500, FC 1:50-1:100

Form

Liquid

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Modification

Unmodified

Molecular Weight

61kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Isotype

IgG

Available Sizes

Frequently Asked Questions

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