CDC7 (17K15) Rabbit Monoclonal Antibody

Product Specifications

Background

DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins) . Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylate MCM2 and MCM3.

Product Name Alternative

Cell division cycle 7-related protein kinase, EC 2.7.11.1, CDC7-related kinase, HsCdc7, huCdc7, CDC7, CDC7L1; Hsk1 ; CDC7 kinase

Gene Name

CDC7

Gene ID

8317

Swiss Prot

O00311

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Monoclonal

Conjugation

Unconjugated

Applications

WB, FC

Purification

Affinity purification

Dilution

WB 1:500-1:2000, FC 1:20-1:100

Form

Liquid

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.