PRAS40 Rabbit Monoclonal Antibody
Product Specifications
Background
PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.
Product Name Alternative
AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate
Gene Name
AKT1S1
Gene ID
84335
Swiss Prot
Q96B36
Host
Rabbit
Reactivity
Human, Rat
Clonality
Monoclonal
Conjugation
Unconjugated
Applications
WB, IP
Field of Research
Cell Biology
Purification
Affinity Purification
Dilution
WB 1:500-1:1000, IP 1:20-1:50
Form
Liquid
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Modification
Unmodified
Molecular Weight
Calculated MW: 27 kDa; Observed MW: 40 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.
Isotype
IgG
Available Sizes
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