AKT Rabbit Monoclonal Antibody
Product Specifications
Background
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Product Name Alternative
AKT1
Gene Name
AKT1/AKT2/AKT3
Gene ID
207/208/10000
Swiss Prot
P31749/P31751/Q9Y243
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster
Clonality
Monoclonal
Conjugation
Unconjugated
Applications
WB, IHC, ICC/IF, IP
Field of Research
Signal Transduction
Purification
Affinity Purification
Dilution
WB 1:500-1:1000, IHC 1:50-1:100, ICC/IF 1:50-1:200, IP 1:20-1:50
Form
Liquid
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Modification
Unmodified
Molecular Weight
Calculated MW: 56 kDa; Observed MW: 56 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.
Isotype
IgG
Available Sizes
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