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TriMethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Product Name Alternative

H3K9me3; H3 histone; HIST1H3A; Histone cluster 1; H3a

Gene Name

H3-4

Gene ID

8350

Swiss Prot

P68431

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Monoclonal

Conjugation

Unconjugated

Applications

WB, IHC, ICC/IF

Field of Research

Epigenetics and Nuclear Signaling

Purification

Affinity Purification

Dilution

WB 1:500-1:1000, IHC 1:50-1:100, ICC/IF 1:50-1:200

Form

Liquid

Buffer

Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Modification

Methylated

Molecular Weight

Calculated MW:15 kDa; Observed MW: 17 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Product Datasheet

https://assets.enkilife.com/files/antibody/AMRe03911_Enkilife_EN.pdf

Isotype

IgG

Available Sizes

Curated Selection

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