Phospho-Src (Tyr530) Rabbit Monoclonal Antibody

Product Specifications

Background

Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2) .

Product Name Alternative

SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

Gene Name

SRC

Gene ID

6714

Swiss Prot

P12931

Host

Rabbit

Reactivity

Rat

Clonality

Monoclonal

Conjugation

Unconjugated

Applications

WB, IP

Field of Research

Signal Transduction

Purification

Affinity Purification

Dilution

WB 1:500-1:1000, IP 1:20-1:50

Form

Liquid

Buffer

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Modification

Phosphorylated

Molecular Weight

Calculated MW: 60 kDa; Observed MW: 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Isotype

IgG

Available Sizes

Curated Selection

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