Phospho-Src (Tyr530) Rabbit Monoclonal Antibody
Product Specifications
Background
Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2) .
Product Name Alternative
SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
Gene Name
SRC
Gene ID
6714
Swiss Prot
P12931
Host
Rabbit
Reactivity
Rat
Clonality
Monoclonal
Conjugation
Unconjugated
Applications
WB, IP
Field of Research
Signal Transduction
Purification
Affinity Purification
Dilution
WB 1:500-1:1000, IP 1:20-1:50
Form
Liquid
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Modification
Phosphorylated
Molecular Weight
Calculated MW: 60 kDa; Observed MW: 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.
Isotype
IgG
Available Sizes
Frequently Asked Questions
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