Fluo-4 AM (2mM), Calcium Ion Fluorescent Probe

1. If a culture medium containing serum is used, the esterase in the serum will decompose the AM ester body, thereby reducing the effect of Fluo-4, AM ester entering the cells. In addition, a culture medium containing phenol red will slightly increase the background value. Before adding the working solution, the residual culture medium should be removed as much as possible.2. All fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching.3. Fluo-4, AM ester is easy to absorb moisture. After taking it out of the refrigerator, please make sure it is in a dry environment and put it at room temperature before opening. Since the reagent is very small, please centrifuge it briefly before opening to ensure that the powder falls to the bottom of the tube.4. Fluo-4, AM ester is easily decomposed when in contact with water. If it cannot be used up all at once, it is recommended to store the stock solution in small portions.