JC-1 Mitochondrial Membrane Potential Assay Kit

Store at -20°C for 12 months, keep away from light.Component B can also be stored at 4°C. To avoid repeated freezing and thawing, it is recommended to devide components A and C into small packages.

1. All fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching.2. To avoid repeated freezing and thawing, this product can be divided into small quantities.3. Before use, please centrifuge the product to the bottom of the tube and then proceed with subsequent experiments.4. JC-1 (100x in DMSO) will solidify and stick to the bottom, wall or lid of the centrifuge tube at a lower temperature. It can be incubated in a 20-25°C water bath for a while until it is completely melted before use.5. When preparing the JC-1 staining working solution, you must first fully dissolve the JC-1 (100x in DMSO) provided in the kit with sterile diH2O and then add the 10x Assay Buffer. Do not prepare the 1x Assay Buffer first and then add JC-1 (100x in DMSO), as this will make it difficult for JC-1 to fully dissolve, which will seriously affect subsequent detection. 6. When washing with 1x Assay Buffer after JC-1 staining, keep the 1x Assay Buffer at around 4°C. This will achieve better washing results.7. After JC-1 staining and washing, try to complete the subsequent test within 30 minutes. Keep in ice bath before testing.8. This product is For Research Use Only, Not for Diagnostic Use.