β-Gal Senescence Staining Kit

Cell senescence refers to the gradual decline in cell proliferation, differentiation capacity, and physiological functions that occur over time during life activities. Under normal circumstances, the body eliminates senescent cells, while those remaining may accumulate, potentially causing low-level inflammation or further inducing tissue degeneration and carcinogenesis. Key characteristics of cellular senescence include morphological changes, cell cycle arrest, senescence-associated secretory phenotype, macromolecular damage, and metabolic disorders. Currently, the most specific marker for identifying senescence is senescence-associated β-galactosidase (SA-β-Gal), which accumulates with cellular aging and is absent in pre-senescence cells, quiescent cells, and terminally differentiated cells. This kit utilizes the fact that β-Gal in senescent cells can hydrolyze 5-bromo-4-chloro-3-indolyl-β-D-pentose galactoside (X-gal) into a blue substance at pH 6.0, enabling observation of cellular or tissue senescence under optical microscopy.

Product Specifications

Applications

Cellular Senescence

Field of Research

Cellular Senescence

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C for 12 months, keep away from light

Notes

1.Before use, centrifuge the product to the tube bottom immediately before proceeding with subsequent experiments. 2. The senescence β-galactosidase detection relies on specific pH conditions. Since high CO2 concentrations in CO2 incubators can affect the pH of staining working solution, 37°C incubation should not be performed in CO2 incubators. 3. X-Gal solution may freeze when stored at-20°C °C or 4°C °C. It can be fully thawed by 2-5 minutes at room temperature or in a 37°C water bath with gentle shaking. For aliquoting the solution or preparing staining working solutions, use polypropylene (PP) or glass materials instead of polystyrene (PS) containers such as cell culture plates or serum pipettes. 4. Minor sediment may appear at the bottom of tube B of β-galactosidase staining solution. Shake vigorously to ensure complete dissolution before staining. 5. This kit is theoretically applicable to frozen sections but not paraffin sections. For staining, refer to relevant literature and conduct preliminary experiments to optimize protocols. 6. β-galactosidase staining fixative contains toxic and corrosive substances. For safety, wear lab coats and disposable gloves to avoid direct contact with human skin or inhalation. 7. This product is strictly for research purposes only and must not be used for clinical diagnosis or treatment.