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Viability/Cytotoxicity Assay Kits for Animal Cells

The Calcein AM/EthD-I Dual Fluorescence Assay Kit is a dual-labeled reagent for detecting viable and dead animal cells. The kit uses two probes to measure intracellular lysis enzyme activity and plasma membrane integrity respectively, providing a more accurate assessment of cell viability. Compared to the conventional Trypan blue method, this kit offers faster detection, enhanced safety, and higher sensitivity. It is compatible with fluorescence microscopy, flow cytometers, microplate readers, and other fluorescence detection systems for most eukaryotic mammalian cells, including certain tissue cultures with adherent cell nuclei. However, it is not suitable for fungal or yeast cells.

Product Specifications

Applications

Viability, Cytotoxicity

Field of Research

Cell Viability and Cytotoxicity

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C for 12 months, keep away from light

Notes

1. Before use, centrifuge the product to the tube bottom immediately before proceeding with subsequent experiments. 2. Phenol red or serum may interfere with the detection of this kit. 3. Fluorescent dyes are prone to quenching. During operation, take precautions against light exposure to minimize fluorescence quenching. 4. For your safety and health, wear lab coats and disposable gloves during handling. 5. This product is for scientific research purposes only and must not be used for clinical diagnosis or treatment.

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