Viability/Cytotoxicity Assay Kits for Bacteria Cells

The bacterial viability and toxicity detection kit contains two fluorescent dyes: NucGreen (a green nucleic acid dye that stains both live and dead bacteria) and EthD-III (a red nucleic acid dye that specifically stains dead bacteria with damaged cell membranes) . When mixed appropriately, bacteria with intact cell membranes appear green, while those with damaged membranes show distinct green and red fluorescence signals under different detection channels. This kit is suitable for staining most bacterial types and can be analyzed using either fluorescence microscopy or flow cytometry. The excitation and emission spectra of NucGreen and EthD-III are detailed in the product specifications. A common standard for bacterial viability assessment is growth measurement, which evaluates a bacterium's ability to multiply in nutrient-rich culture media. The kit's results demonstrate excellent consistency with growth measurements obtained from both liquid and solid culture media.