EdU Cell Proliferation Assay Kit (Fluorescein 594, Red)

Cell proliferation detection is a fundamental experimental method for assessing cell health, genetic toxicity, and the efficacy of anti-tumor drugs. The most accurate method for detecting cell proliferation is the BrdU method. The EdU method is a revolutionary breakthrough over the BrdU method. EdU (5-ethynyl-2'-deoxyuridine) is a pyrimidine analog that integrates into DNA during the DNA synthesis phase. The detection is based on a "click" reaction, a covalent reaction catalyzed by copper between azide compounds and alkynes. The EdU labeling method is rapid and efficient, easy to use. It only requires formaldehyde fixation and Triton X-100 permeabilization to allow the detection reagent to enter the cells. A small amount of azide dye can effectively label the integrated EdU. In contrast, the BrdU method requires DNA denaturation (such as acid denaturation, heat denaturation, or DNase digestion) to expose BrdU for antibody binding. This kit includes all the components required for EdU detection and can be used for the proliferation detection of in vitro cultured cells.