FITC-Annexin V/PI Apoptosis Kits (Green/Red)

The FITC -Annexin V/PI Apoptosis Detection Kit provides a rapid and simple method to Detect apoptosis by labeling early apoptotic cells (Far-Red) and necrotic or late apoptotic cells (red) . Annexin V is a Ca²⁺-dependent phospholipid-binding protein with a molecular weight of 35–36 kDa that selectively binds to phosphatidylserine (PS) . In normal cells, PS is located on the inner side of the plasma membrane. During early apoptosis, PS is translocated to the outer surface of the cell membrane and exposed to the extracellular environment. By binding FITC -labeled Annexin V to the exposed PS, this hallmark of apoptosis can be directly detected using flow cytometry or fluorescence microscopy. Propidium Iodide (PI) is a DNA-binding dye that stains the nuclei of necrotic or late apoptotic cells that have lost membrane integrity, emitting red fluorescence upon excitation.