Recombinant Human Wnt3a V2

Product Specifications

Background

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity) . Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) .

Product Name Alternative

MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a

Accession Number

P56704

Host

Human Cells

Endotoxin

<0.01 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Lyophilized

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

105.7 kDa

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Formulation

Lyophilized from a 0.2 μm filtered solution of 10mM PB, 5% Sucrose, 0.01% Tween 80, pH7.4.

Construct

Recombinant Human Protein Wnt-3a is produced by our Mammalian expression system and the target gene encoding Ser19-Lys352 is expressed with a fusion design at the N-terminus.