Recombinant Mouse HGF (C-6His)

HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60/xa0kDa alpha /xa0and 30 kDa beta /xa0chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. /xa0It can also alter epithelium morphology by the induction of nectin‑1 alpha /xa0ectodomain shedding, an adhesion protein component of adherens junctions/xa0.

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Recombinant Mouse Hepatocyte Growth Factor is produced by our Mammalian expression system and the target gene encoding Gln33-/xadLeu728 is expressed with a 6His tag at the C-terminus.