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Recombinant Human HMGB1 (Truncated)

Product Specifications

Background

High mobility group protein B1 is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus. As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile, it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte, T cell, dendritic cell activities in inflammatory responses.

Product Name Alternative

High Mobility Group Protein B1; High Mobility Group Protein 1; HMG-1; HMGB1; HMG1

Accession Number

P09429

Host

E.coli

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Lyophilized

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

10 KDa

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50mM HEPES-Na, 500mM NaCl, 0.6mM DTT, pH 7.9.

Construct

Recombinant Human High Mobility Group Protein B1 is produced by our E.coli expression system and the target gene encoding Pro92-Val176 is expressed.

Species

Human

Available Sizes

Frequently Asked Questions

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