Recombinant Human HLA-A*0201 HPV16 E7 complex Protein (C-10His)

Product Specifications

Background

The HPV16 E7 protein plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. E7 protein has both transforming and trans-activating activities. It plays also a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha.

Product Name Alternative

HLA-A*0201 HPV16 E7 complex Protein

Accession Number

P61769&P01892

Host

Human Cells

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Lyophilized

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

49.1 KDa

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, 0.06% Tween 80, pH7.4.

Construct

Recombinant Human HLA-A*0201 HPV16 E7 complex Protein is produced by our Mammalian expression system and the target gene encoding (YMLDLQPET) &Ile21-Met119&Gly25-Ile308 (Ala269Val) is expressed with a 10His tag at the C-terminus.