Recombinant Human HLA-A*0201 WT-1 complex Protein (C-10His)

Product Specifications

Background

The WT1 protein plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation.

Product Name Alternative

Accession Number

P61769&P01892

Host

Human Cells

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Lyophilized

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

49.1 KDa

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 5% Mannitol, 0.01% Tween 80, pH 7.4.

Construct

Recombinant Human HLA-A*0201 WT-1 complex Protein is produced by our Mammalian expression system and the target gene encoding (RMFPNAPYL) &Ile21-Met119&Gly25-Ile308 (Ala269Val) is expressed with a 10His tag at the C-terminus.