Recombinant Vibriocholerae Toxin B
Product Specifications
Background
Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. It is responsible for the massive, watery diarrhea characteristic of cholera infection. Cholera enterotoxin subunit B (CTXB) pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
Product Name Alternative
Cholera enterotoxin subunit B; Cholera enterotoxin B chain; Cholera enterotoxin gamma chain; Choleragenoid; ctxB; toxB
Accession Number
P01556
Host
E.coli
Endotoxin
<1 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE
Form
Lyophilized
Reconstitution
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular Weight
11.8 KDa
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Formulation
Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 200mM NaCl, pH 8.0.
Construct
Recombinant Vibriocholerae Toxin B is produced by our E.coli expression system and the target gene encoding Thr22-Asn124 is expressed.
Species
Vibrio cholerae
Available Sizes
Frequently Asked Questions
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