Recombinant Human sCD4 (C-Fc)

CD4 is an approximately 55/xa0kDa type/xa0I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T/xa0lymphocytes. It is a standard phenotype marker for the identification of T/xa0cell populations. Mature human CD4 consists of a 371/xa0amino acid extracellular region containing four immunoglobulin-like domains, a 22/xa0aa transmembrane segment, and a 40/xa0aa cytoplasmic domain. CD4 is expressed along with CD8 on double positive T/xa0cells during their development in the thymus. CD4 binds directly to MHC class/xa0II molecules on antigen presenting cells (10) . This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1.

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Recombinant Human T-cell Surface Glycoprotein CD4 is produced by our Mammalian expression system and the target gene encoding Lys26-Trp390 is expressed with a human IgG1 Fc tag at the C-terminus.