Recombinant Human ER alpha (N-6His)

Product Specifications

Background

Estrogen Receptor is a major ligand-activated transcription factor belonging to the nuclear hormone receptor superfamily. Estrogen Receptor is composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but they also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5 UTRs and use different promoters.

Product Name Alternative

Estrogen Receptor; ER; ER-Alpha; Estradiol Receptor; Nuclear Receptor Subfamily 3 Group A Member 1; ESR1; ESR; NR3A1; Estrogen Receptor Alpha; ER alpha; estrogen receptor alpha

Accession Number

P03372

Host

E.coli

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Lyophilized

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

14.38 KDa

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM Glycine-HCl, 8% Sucrose, 0.05% Tween 80, pH 3.5.

Construct

Recombinant Human Estrogen Receptor Alpha is produced by our E.coli expression system and the target gene encoding Met1-Gln116 is expressed with a 6His tag at the N-terminus.