Recombinant Human EIF4EBP2 (N-6His)

Product Specifications

Background

Eukaryotic Translation Initiation Factor 4E-Binding Protein 2 (EIF4EBP2) is a member of the Eukaryotic Translation Initiation Factor 4E Binding Protein Family. EIF4EBP2 regulates eIF4E activity by preventing its assembly into the eIF4F complex, mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway. This regulation of is associated to cell proliferation, cell differentiation and viral infection. Phosphorylated EIF4EBP2 on serine and threonine residues in response to insulin, EGF and PDGF.

Product Name Alternative

Eukaryotic Translation Initiation Factor 4E-Binding Protein 2; 4E-BP2; eIF4E-Binding Protein 2; EIF4EBP2

Accession Number

Q13542

Host

E.coli

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Lyophilized

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

15.1 KDa

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

Construct

Recombinant Human Eukaryotic Translation Initiation Factor 4E-Binding Protein 2 is produced by our E.coli expression system and the target gene encoding Met1-Ile120 is expressed with a 6His tag at the N-terminus.