Recombinant Human B3GNT1 (C-6His)

Product Specifications

Background

N-Acetyllactosaminide β-1,3-N-Acetylglucosaminyltransferase (B3GNT1) is a member of the β-1,3-N-Acetylglucosaminyltransferase family. B3GNT1 is a single-pass type II membrane protein and widely expressed in many tissues. B3GNT1 can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans. B3GNT1 is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen. It can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans.

Product Name Alternative

N-Acetyllactosaminide Beta-1; 3-N-Acetylglucosaminyltransferase; I-Beta-1; 3-N-Acetylglucosaminyltransferase; iGnT; Poly-N-Acetyllactosamine Extension Enzyme; UDP-GlcNAc:BetaGal Beta-1; 3-N-Acetylglucosaminyltransferase 1; B3GNT1; B3GNT6

Accession Number

O43505

Host

Human Cells

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Lyophilized

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

43.4 KDa

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl,150mM NaCl, pH 7.4.

Construct

Recombinant Human N-Acetyllactosaminide Beta-1,3-N-Acetylglucosaminyltransferase is produced by our Mammalian expression system and the target gene encoding Asp43-Cys415 is expressed with a 6His tag at the C-terminus.