Recombinant Human CD73 (AAH65937.1, C-Fc)
Product Specifications
Background
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5-nucleotidase family. CD73 is an ecto 5Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA) . The recombinant CD73 lacking GPI anchor is secreted as a monomer.
Product Name Alternative
5-Nucleotidase; 5-NT; Ecto-5-Nucleotidase; CD73; NT5E; NT5; NTE
Accession Number
AAH65937.1
Host
Human Cells
Endotoxin
<1 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE
Form
Liquid
Molecular Weight
84.6 KDa
Shipping Conditions
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
Construct
Recombinant Human 5-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a human IgG1 Fc tag at the C-terminus.
Species
Human
Available Sizes
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