Recombinant Human RNASE 1 (C-6His)

Product Specifications

Background

Ribonuclease Pancreatic is a secreted enzyme that belongs to the pancreatic ribonuclease family. RNASE1 is an endonuclease that cleaves internal phosphodiester RNA bonds on the 3-side of pyrimidine bases. RNASE1 prefers poly (C) as a substrate and hydrolyzes 2,3-cyclic nucleotides, with a pH optimum near 8.0. RNASE1 acts on single stranded and double stranded RNA.

Product Name Alternative

Ribonuclease Pancreatic; HP-Rnase; RIB-1; RNase UpI-1; Ribonuclease 1; RNase 1; Ribonuclease A; RNase A; RNASE1; RIB1; RNS1

Accession Number

P07998

Host

Human Cells

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Liquid

Molecular Weight

15.6 KDa

Shipping Conditions

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Glycerol, pH 7.4.

Construct

Recombinant Human Ribonuclease Pancreatic is produced by our Mammalian expression system and the target gene encoding Lys29-Thr156 is expressed with a 6His tag at the C-terminus.