Recombinant Human NADK (N-6His)

Product Specifications

Background

NAD Kinase (NADK) is an enzyme that belongs to the NAD Kinase family. It is a widely expressed enzyme, but it is not detected in skeletal muscle. NADK converts Nicotinamide Adenine Dinucleotide (NAD+) into NADP+, through phosphorylating the NAD+ coenzyme. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. The structure of the NADK from the archaean Archaeoglobus fulgidus has been determined.

Product Name Alternative

NAD Kinase; Poly (P) /ATP NAD Kinase; NADK

Accession Number

AAH01709.1

Host

E.coli

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Liquid

Molecular Weight

44.4 KDa

Shipping Conditions

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM His-HCl, 5% Sucrose, 10% Glycerol, 150 mM NaCl, 0.02%Tween80, 1 mM DTT, pH3.0.

Construct

Recombinant Human NAD Kinase is produced by our E.coli expression system and the target gene encoding Ser64-Gly446 is expressed with a 6His tag at the N-terminus.