Recombinant K. pneumoniae NEO
Product Specifications
Background
Aminoglycoside 3-phosphotransferase (APH (3) ), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH (3) is primarily found in certain species of gram-positive bacteria.
Product Name Alternative
Aminoglycoside 3-phosphotransferase; APH (3) -II; APH (3) II; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo
Accession Number
P00552
Host
E.coli
Endotoxin
<1 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE
Form
Liquid
Molecular Weight
29 KDa
Shipping Conditions
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 6%Trehalose, 4%Mannitol, 0.05%Tween80, PH8.0.
Construct
Recombinant Klebsiella Pneumoniae Aminoglycoside 3-phosphotransferase is produced by our E.coli expression system and the target gene encoding Met1-Phe264 is expressed.
Species
Klebsiella pneumoniae
Available Sizes
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