Recombinant Human GSTZ1 (C-6His)

Product Specifications

Background

Maleylacetoacetate Isomerase (MAAI) belongs to the Glutathione S-Transferase super-family. MAAI encodes multifunctional enzymes in the detoxification of electrophilic molecules by conjugation with glutathione, for example, mutagens, carcinogens and several therapeutic drugs. MAAI is a bifunctional protein with low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. MAAI can catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid. But it has minimal glutathione-conjugating activity with 7-chloro-4-nitrobenz-2-oxa-1, ethacrynic acid 3-diazole and maleylacetoacetate isomerase activity.

Product Name Alternative

Maleylacetoacetate Isomerase; MAAI; GSTZ1-1; Glutathione S-Transferase Zeta 1; GSTZ1

Accession Number

O43708

Host

E.coli

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Liquid

Molecular Weight

25.18 KDa

Shipping Conditions

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Formulation

Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 1mM DTT, pH 8.0.

Construct

Recombinant Human Glutathione S-Transferase Zeta 1 is produced by our E.coli expression system and the target gene encoding Met1-Ala216 is expressed with a 6His tag at the C-terminus.