Recombinant Human ECH1 (N-6His)

Product Specifications

Background

Human delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase (ECH1) is a member of the hydratase/isomerase superfamily and contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. The rat orthologlocalizes to the matrix of both the peroxisome and mitochondria.It can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 plays an important role in the auxiliary step of the fatty acid beta-oxidation pathway.

Product Name Alternative

Delta (3;5) -Delta (2;4) -dienoyl-CoA isomerase; mitochondrial; ECH1

Accession Number

Q13011

Host

E.coli

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Liquid

Molecular Weight

34.5 KDa

Shipping Conditions

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 8% Trehalose, 0.05% tween80, pH8.5.

Construct

Recombinant Human Delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase, Mitochondrial is produced by our E.coli expression system and the target gene encoding Thr34-Leu328 is expressed with a 6His tag at the N-terminus.