Recombinant Human dUTPase

Product Specifications

Background

Deoxyuridine 5-Triphosphate Nucleotidohydrolase Mitochondrial (dUTPase) belongs to the dUTPase family. dUTPase exits as a homotrimer and is involved in nucleotide metabolism. dUTPase produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. The dUTPase increase in PCR product yield, length and fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity and yield-sensitive applications. dUTPase is specific for dUTP and is critical for the fidelity of DNA replication and repair.

Product Name Alternative

Deoxyuridine 5-Triphosphate Nucleotidohydrolase Mitochondrial; dUTPase; dUTP Pyrophosphatase; DUT

Accession Number

P33316-2

Host

E.coli

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Liquid

Molecular Weight

17.7 KDa

Shipping Conditions

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Construct

Recombinant Human Deoxyuridine 5-Triphosphate Nucleotidohydrolase, Mitochondrial is produced by our E.coli expression system and the target gene encoding Met1-Asn164 is expressed.