Recombinant Human CFD (C-10His)

Product Specifications

Background

Complement factor D, also known as adipsin, is a member of the chymotrypsin family of serine proteases, which plays an essential role in host defense as the rate-limiting enzyme in the alternative pathway of complement activation. Complement factor D activates a convertase (C3bBb) responsible for cleavage of the complement protein C3, which leads to the activation of terminal complement component C5-9 to form the membrane attack complex on microbial or cellular surfaces. It also functions in the regulation of systemic energy balance and physiologic and pathologic processes, including immunity and inflammation.

Product Name Alternative

Complement factor D; CFD; Adipsin; C3 convertase activator; Properdin factor D; DF; PFD

Accession Number

P00746

Host

Human Cells

Endotoxin

<1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Form

Liquid

Molecular Weight

25.8 KDa

Shipping Conditions

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 2mM CaCl2, 5% Trehalose, 50% Glycerol, pH 8.5.

Construct

Recombinant Human Complement Factor D is produced by our Mammalian expression system and the target gene encoding Ile26-Ala253 is expressed with a 10His tag at the C-terminus.