Recombinant Human BDH1 (N-6His)
Product Specifications
Background
D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial (BDH) is a member of the short-chain dehydrogenases/reductases (SDR) family. BDH is localized in the mitochondrion matrix. BDH forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific necessity for phosphatidylcholine for optimal enzymatic activity. BDH catalyzes the interconversion of acetoacetate and (R) -3-hydroxybutyrate, the 2 main ketone bodies formed during fatty acid catabolism.
Product Name Alternative
D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial; BDH; 3-Hydroxybutyrate Dehydrogenase; BDH
Accession Number
Q02338
Host
E.coli
Endotoxin
<1 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE
Form
Liquid
Molecular Weight
40.3 KDa
Shipping Conditions
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Formulation
Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 1mM DTT, pH 7.5.
Construct
Recombinant Human D-beta-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 is produced by our E.coli expression system and the target gene encoding Met1-Arg343 is expressed with a 6His tag at the N-terminus.
Species
Human
Available Sizes
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