CD3 zeta (pY153) Antibody
Mouse monoclonal antibody to CD3 zeta
Product Specifications
Product Name Alternative
CD3 zeta chain, CD247, T3Z, TCRz, IMD25
UniProt
P20963
Reactivity
Human, Mouse
Immunogen
A phospho specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH
Target
CD3 zeta (pY153)
Clonality
Monoclonal
Clone
EM-17
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Western blotting: Recommended dilution: 2 - 5 μg/ml; positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended. Flow cytometry: Intracellular staining; recommended dilution: 1-4 μg/ml; positive control: Jurkat cells treated with pervanadate.
Tested Applications
FC, WB
Preservative
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Isotype
Mouse IgG1
Entrez
919
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