IL-10 (3-8) monoclonal antibody
Product Specifications
Background
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Structure Composition
Mouse IgG, 0.6mg/ml in PBS with 0.05% Proclin300, 50% glycerol, 0.2% BSA, pH7.2.
Product Name Alternative
IL-10; Cytokine synthesis inhibitory factor (CSIF) ; Interleukin-10
Swiss Prot
P22301
Reactivity
Human, Rat, Mouse
Immunogen
Recombinant protein of human IL-10.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:1000~1:2000
Purity
The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 21 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IL-10 (3-8) monoclonal antibody detects endogenous levels of IL-10 protein.
Applications Notes
Western blot (WB) analysis of IL-10 (3-8) monoclonal antibody at 1:1000 dilution Lane1:THP-1 whole cell lysate (40ug) Lane2:THP-1 treated with LPS (2ug/ml,6h), BFA (2ug/ml,3h) whole cell lysate (40ug)
Host or Source
Mouse
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
