IL-12 (7-16) monoclonal antibody

Product Specifications

Background

This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.

Structure Composition

Mouse IgG, 1.2mg/ml in PBS with 0.05% Proclin300, 50% glycerol, pH7.2.

Product Name Alternative

Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40) ; IL-12 subunit p40; NK cell stimulatory factor chain 2 (NKSF2) ; IL12B; NKSF2

Swiss Prot

P29460

Reactivity

Human, Mouse

Immunogen

Recombinant protein of Human IL-12.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:2000~1:10000

Purity

The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 33 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IL-12 (7-16) polyclonal antibody detects endogenous levels of IL-12 protein.

Applications Notes

Western blot (WB) analysis of IL-12 (7-16) monoclonal antibody at 1:2000 dilution Lane1:RAW264.7 whole cell lysate (30ug) Lane2:RAW264.7 treated with LPS (300ng/ml,6h) and BFA (300ng/ml,3h) whole cell lysate (30ug)