PKM1/PKM2 (28-4-11) monoclonal antibody

Product Specifications

Background

Pyruvate kinase is a glycolytic enzyme that catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2) is an alternatively spliced variant of M1 that is expressed during embryonic development. Research studies found that cancer cells exclusively express PKM2. PKM2 is shown to be essential for aerobic glycolysis in tumors, known as the Warburg effect. When cancer cells switch from the M2 isoform to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased. These cells also show decreased tumorigenicity in mouse xenografts. Recent studies showed that PKM2 is not essential for all tumor cells. In the tumor model studied, PKM2 was found to be active in the non-proliferative tumor cell population and inactive in the proliferative tumor cell population.

Structure Composition

Mouse IgG, 0.4mg/ml in PBS with 0.05% Proclin300, 50% glycerol, 0.2% BSA, pH7.2.

Product Name Alternative

Pyruvate kinase PKM; Cytosolic thyroid hormone-binding protein; Opa-interacting protein 3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Threonine-protein kinase PKM2; PKM; OIP3; PK2; PK3; PKM2

Swiss Prot

P14618

Reactivity

Human, Rat, Mouse

Immunogen

Recombinant protein of Human PKM1/PKM2.

Conjugation

Unconjugated

Applications

WB IP

Dilution

WB: 1:1000~1:2000 IP: 1:50~1:200

Purity

The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 65 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PKM1/PKM2 (28-4-11) polyclonal antibody detects endogenous levels of PKM1/PKM2 protein.

Applications Notes

Western blot (WB) analysis of PKM1/PKM2 (28-4-11) monoclonal antibody at 1:1000 dilution Lane1:PKM1 recombinant protein (0.2ug) Lane2:PKM2 recombinant protein (0.2ug) Lane3:A549 whole cell lysate (30ug) Lane4:The Muscle tissue lysate of Mouse (30ug)