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MLKL (phospho-S345) polyclonal antibody

Product Specifications

Background

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process.

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Mixed lineage kinase domain-like protein; hMLKL

Swiss Prot

Q9D2Y4

Reactivity

Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human MLKL.

Conjugation

Unconjugated

Applications

WB, IF

Dilution

WB,1:500 - 1:1000|IF,1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

54 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MLKL (phospho-S345) polyclonal antibody detects endogenous levels of MLKL protein only when phosphorylated at S345.

Applications Notes

Western blot analysis of MLKL (Phospho-S345) expression in mouse kidney (A), rat kidney (B) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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