Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12)

Product Specifications

Background

Monomethyl auristatin E (MMAE) is a synthetic derivative of dolastatin 10 and functions as a potent mitotic inhibitor by inhibiting tubulin polymerization. MMAE is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several cancer types. Anti-MMAE antibody is a rabbit monoclonal antibody specially react with MMAE without MMAF, which is more sensitive than mouse antibody. The anti-MMAE antibody is a useful reagent in PK assay to determine conjugated antibodies.

Structure Composition

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Immunogen

MMAE-BSA

Conjugation

Unconjugated

Applications

ELISA

Dilution

ELISA 0.06-125 ng/mL

Purity

Protein A purified / Protein G purified

Modification

Unmodified

Storage Conditions

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Specifically recognizes MMAE and does not recognize MMAF.

Applications Notes

Immobilized Disitamab Vedotin (RC48) at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) with a linear range of 0.06-2 ng/mL (QC tested) .