Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H05)

Product Specifications

Background

Monomethyl auristatin E (MMAE) is a synthetic derivative of dolastatin 10 and functions as a potent mitotic inhibitor by inhibiting tubulin polymerization. MMAE is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several cancer types. Anti-MMAE antibody is a rabbit monoclonal antibody. The anti-MMAE antibody is a useful reagent in PK assay to determine conjugated antibodies.

Structure Composition

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Immunogen

MMAE-BSA

Conjugation

Unconjugated

Applications

ELISA

Dilution

ELISA 0.03-31 ng/mL

Purity

Protein A purified / Protein G purified

Modification

Unmodified

Storage Conditions

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Specifically rSpecifically recognizes MMAE and does not recognize MMAF.

Applications Notes

Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H05) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Human Her2, His, Avitag, premium grade at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (QC tested) .