Calmodulin (Phospho-T80/S82) Polyclonal Antibody

Product Specifications

Background

Calmodulin is a ubiquitously expressed small protein mediating many cellular effects such as short-term and long-term memory, nerve growth, inflammation, apoptosis, muscle contraction and intracellular movement. Upon binding of four Ca2+ ions, calmodulin undergoes conformational changes, allowing this complex to bind to and activate many enzymes including protein kinases, protein phosphatases, ion channels, Ca2+ pumps, nitric oxide synthase, inositol triphosphate kinase, and cyclic nucleotide phosphodiesterase. Since calmodulin binds Ca2+ in a cooperative fashion, small changes in cytosolic Ca2+ levels lead to large changes in the level of active calmodulin and its target proteins.

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CALM; CAM; CAM1; CAM2; CAMB; CALML2; CAM3; CAMC; CAMIII; Calmodulin; CaM

Swiss Prot

P62158

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T80/S82 of human Calmodulin protein.

Conjugation

Unconjugated

Applications

WB IF/ICC IHC

Dilution

WB: 1:500-1:1000 IF/ICC: 1:100-1:500 IHC: 1:100-1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .