MMP-13 (L22) polyclonal antibody

The matrix metalloproteinases (MMP) are a family ofpeptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibroPVRL1, laminin and proteoglycan. Transcriptionof MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB) . MMP catalysis requires both calcium and zinc.MMP-13 (also designated collagenase-3) is produced bybreast carcinomas and degrades collagen types I, II andIII. MMP-13 has wide substrate specificity, and its physiologic expression is limited to situations in which rapidand effective remodeling of collagenous ECM takesplace, such as fetal bone development and adult bone remodeling

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of MMP-13 (L22) pAb at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:H1792 whole cell lysate (40ug)