MMP-13 (L22) polyclonal antibody
Product Specifications
Background
The matrix metalloproteinases (MMP) are a family ofpeptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibroPVRL1, laminin and proteoglycan. Transcriptionof MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB) . MMP catalysis requires both calcium and zinc.MMP-13 (also designated collagenase-3) is produced bybreast carcinomas and degrades collagen types I, II andIII. MMP-13 has wide substrate specificity, and its physiologic expression is limited to situations in which rapidand effective remodeling of collagenous ECM takesplace, such as fetal bone development and adult bone remodeling
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide,50% glycerol, pH7.2
Product Name Alternative
Collagenase 3; Matrix metalloproteinase-13 (MMP-13) ; MMP13
Swiss Prot
P45452
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to Human MMP-13.
Conjugation
Unconjugated
Applications
WB, IHC, IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 55, 40 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MMP-13 (L22) polyclonal antibody detects endogenouslevels of ~55 kDa latent or the ~40 kDa active forms ofMMP-13 protein.
Applications Notes
Western blot (WB) analysis of MMP-13 (L22) pAb at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:H1792 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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