Myc-Tag mouse monoclonal antibody-HRP

Product Specifications

Background

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da) . It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Structure Composition

Mouse IgG1, 1.5mg/ml in PBS with 0.05% proclin300, 50% glycerol, pH7.2

Product Name Alternative

Myc

Reactivity

Transfected

Immunogen

Synthetic peptide Myc.

Conjugation

HRP

Applications

Dilution

WB: 1:5000~1:20000

Purity

The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Myc-Tag mAb-HRP detects over-expressed or recombinant proteins containing the myc epitope tag.

Applications Notes

Western blot (WB) analysis of Myc-tag mAb at 1:5000 dillution Lane1:HEK293F whole cell lysate Lane2:HEK293F whole cell lysate, transfected (M-Myc) Lane3:HEK293F whole cell lysate, transfected (N-Myc)