Mcherry-Tag mouse monoclonal antibody-HRP
Product Specifications
Background
MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0.5 of 15 minutes, allowing it to be visualised soon after translation.
Structure Composition
Mouse IgG1, 0.75mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
MCherry
Reactivity
Transfected
Immunogen
Synthetic peptide mCherry-Tag.
Conjugation
HRP
Applications
WB
Dilution
WB: 1:2000~1:10000
Purity
The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Mcherry-Tag mAb-HRP detects over-expressed or recombinant proteins containing the mcherry epitope tag.
Applications Notes
Western blot (WB) analysis of mcherry-tag mAb-HRP at 1:5000 dillution Lane1:HEK293F whole cell lysate, transfected (mcherry) Lane2:HEK293F whole cell lysate
Host or Source
Mouse
Available Sizes
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