His-Tag mouse monoclonal antibody

Product Specifications

Background

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Reactivity

Transfected

Immunogen

Synthetic peptide His-tag.

Conjugation

Unconjugated

Applications

WB IHC IF IP

Dilution

WB: 1:20000~1:100000 IHC/IF: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

His-tag mAb detects over-expressed or recombinant proteins containing the 6xHis epitope tag.

Applications Notes

Western blot (WB) analysis of His-tag mAb at 1:100000 dillution Lane1:HEK293F whole cell lysate Lane2:HEK293F whole cell lysate, transfected (C-His) .