Flag-Tag mouse monoclonal antibody
Product Specifications
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag, ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK
Reactivity
Transfected
Immunogen
Synthetic peptide Flag-tag.
Conjugation
Unconjugated
Applications
WB IHC IF IP
Dilution
WB: 1:20000~1:100000 IHC/IF: 1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Flag-Tag mAb detects over-expressed or recombinant proteins containing the Flag epitope tag.
Applications Notes
Western blot (WB) analysis of Flag-Tag mAb at 1:100000 dillution Lane1:HEK293F whole cell lysate , untransfected Lane2:HEK293F whole cell lysate , transfected (M-Flag) Lane3:HEK293F whole cell lysate , transfected (N-Flag) Lane4:HEK293F whole cell lysate , transfected (C-Flag)
Host or Source
Mouse
Available Sizes
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